Scholarly Activity > Publications
Peer-Reviewed Publications
My first full-time job when I finished my undergraduate education was working in a Pathology research lab at Case Western Reserve University. Our lab was developing an enzymatic amplification system for flow cytometry immunostaining. The process of flow cytometry involves tagging molecules of interest with fluorescent markers, so that tagged cells will fluoresce when run in a diluted stream past a laser of a specific wavelength. This is usually done by attaching a fluorescent molecule (perhaps a group containing phosphorus) to an antibody that preferentially seeks out the molecule of interest.
One of the biggest problems with this technique is non-specific binding, or in other words, fluorescing antibodies that aren't attached to the right molecule or aren't attached to any molecule and just can't be washed away. We developed a technique involving a secondary antibody that preferentially seeks out a primary antibody which seeks out the molecule of interest. We perfected the two-stage process for several clinically relevant tests and were able to achieve 10-100x signal amplification, allowing us to pick signal out of what had previously just been noise.
I had the honor of coauthoring two papers:
One of the biggest problems with this technique is non-specific binding, or in other words, fluorescing antibodies that aren't attached to the right molecule or aren't attached to any molecule and just can't be washed away. We developed a technique involving a secondary antibody that preferentially seeks out a primary antibody which seeks out the molecule of interest. We perfected the two-stage process for several clinically relevant tests and were able to achieve 10-100x signal amplification, allowing us to pick signal out of what had previously just been noise.
I had the honor of coauthoring two papers:
D Cyclins in CD5+ B-Cell Lymphoproliferative Disorders
Cyclin D1 and Cyclin D2 Identify Diagnostic Groups and Cyclin D1 Correlates With ZAP-70 Expression in Chronic Lymphocytic Leukemia
Howard J. Meyerson, Grayden MacLennan, William Husel, William Tse, Hillard M. Lazarus, & David Kaplan (2006)
American Journal of Clinical Pathology, 125(2), 241-250.
Cyclin D1 and Cyclin D2 Identify Diagnostic Groups and Cyclin D1 Correlates With ZAP-70 Expression in Chronic Lymphocytic Leukemia
Howard J. Meyerson, Grayden MacLennan, William Husel, William Tse, Hillard M. Lazarus, & David Kaplan (2006)
American Journal of Clinical Pathology, 125(2), 241-250.
We analyzed protein expression of cyclin D1, cyclin D2, and cyclin D3 using high-resolution enzymatic amplification staining and flow cytometry in the neoplastic cells from 80 patients with CD5+ B-cell lymphoproliferative disorders. The D cyclins were expressed differentially in chronic lymphocytic leukemia (CLL), prolymphocytic leukemia (PLL), and mantle cell lymphoma (MCL) with strong staining of cyclin D1 and D2 in MCL, strong staining of cyclin D1 but weak staining of cyclin D2 in 4 of 5 PLLs, and low-level staining for both cyclins in most CLLs. No correlation between cyclin D1 and D2 and growth rates or CD38 expression was observed. However, cyclin D1 levels were significantly higher in ZAP-70+ CLL cases, although no association between ZAP-70 and cyclin D2 was detected. The results indicate that flow cytometric analysis of D cyclins may help in classification of CD5+ B-cell lymphoproliferative disorders.
D Cyclins in Lymphocytes
David Kaplan, Howard J. Meyerson, William Husel, Kristine Lewandowska & Grayden MacLennan (2005)
Cytometry Part A, 63(1), 1-9.
David Kaplan, Howard J. Meyerson, William Husel, Kristine Lewandowska & Grayden MacLennan (2005)
Cytometry Part A, 63(1), 1-9.
Using high-resolution technology for flow cytometry, we found all three D cyclins in quiescent human peripheral blood lymphocytes. Cyclin D1 was expressed in quiescent and activated cells at levels commensurate with those of actively proliferating tumor cell lines. Cyclin D1 was functional inasmuch as it was complexed with CDK4. In the quiescent cells, cyclin D1 was expressed in the cytoplasm but, after activation, was found in the nucleus.